A Rat Uterus Contractile Substance in Clostridium botulinum Type A

The Inhibitory Effect of Botulinum Toxin Type A on Rat Pyloric Smooth Muscle Contractile Response to Substance P In Vitro

A decrease in pyloric myoelectrical activity and pyloric substance P (SP) content following intrasphincteric injection of botulinum toxin type A (BTX-A) in free move rats have been demonstrated in our previous studies. The aim of the present study was to investigate the inhibitory effect of BTX-A on rat pyloric muscle contractile response to SP in vitro and the distributions of SP and neurokini...

Purification of Clostridium botulinum type A neurotoxin.

production and crystallization of clostridium botulinum type a toxin

introduction: widespread medical usage of botulinum toxins has increased the need for the precise analysis of its biological activity. the aim of this study was production and crystallization of the botulinum type a toxins. methods: in this study, clostridium botulinum was grown and inoculated into the tripticase soya broth culture media, under anaerobic condition and toxin production was monit...

a survey of clostridium botulinum in food poisoning in iran

food-borne botulism is one of the dangerous food poisonings in human in the world. the specimens of 115 patients (serum and stool) with clinical symptoms of botulism, who were inpatient and outpatient were collected at some medical centers in tehran and other areas of iran, between april 1984 to august 1994. in this survey, specimens of 73 patients showed the toxin and spore of c.botulinum. clo...

Molecular construction of Clostridium botulinum type A toxins.

Two Clostridium botulinum type A toxic fractions, named large (L) and medium (M) toxins, were eluted from Sephadex G-200. Sucrose density gradient centrifugation resolved L toxin (2.5 X 10(8) to 3.0 X 10(8) mean lethal doses per mg of N) into two fractions, 19S and 16S. The same procedure performed at pH 8resolved it into three fractions; the heavier two were both nontoxic and hemagglutinin pos...